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Image Search Results
Journal: Journal of visualized experiments : JoVE
Article Title: Establishing a High Throughput Epidermal Spheroid Culture System to Model Keratinocyte Stem Cell Plasticity
doi: 10.3791/62182
Figure Lengend Snippet: Outlines PCR primer sequences used for the detection of select genes involved in neonatal keratinocyte plasticity.
Article Snippet: In a 6-cm dish, transfect spheroid-forming 2D monolayer NHKc cultures with 1 μg pMSCV-IRES-EGFP plasmid vector carrying the enhanced green fluorescent protein (eGFP) gene using the
Techniques: Cell Culture, SYBR Green Assay, Plasmid Preparation